Smooth Muscle Research Centre >> People >> Prof Noel McHale

Prof Noel McHale

Position

Professor

Email Address

noel.mchale@dkit.ie

Address

Room 105
Smooth Muscle Research Centre
Dundalk Institute of Technology
Dublin Road
Dundalk
Ireland

Lab Telephone

0429 370 406

Office Telephone

0429 370 406

Research Interests   My research is currently focused on examining the ionic mechanisms underlying spontaneous activity in lymphatic and urethral smooth muscle. We have characterised the main conductances present in these two tissues at both the whole cell and single channel level using standard patch clamp techniques. We have also assessed the contribution of each of these currents to spontaneous and evoked electrical activity in freshly dispersed single cells and in whole tissue.   Publications   Hollywood MA, Sergeant GP, McHale NG, Thornbury KD. Activation of Ca2+-activated Cl-current by depolarizing steps in rabbit urethral interstitial cells. American Journal of Physiology - Cell Physiology 2003; 285: C327-C333. McCloskey KD, Hollywood MA, Thornbury KD, Ward SM, McHale NG. Kit-like immunopositive cells in sheep mesenteric lymphatic vessels. Cell and Tissue Research 2002; 310: 77-84. Sergeant GP, Thornbury KD, McHale NG, Hollywood MA. Characterization of norepinephrine-evoked inward currents in interstitial cells isolated from the rabbit urethra. American Journal of Physiology - Cell Physiology 2002; 283: C885-C894. Sergeant GP, Hollywood MA, McCloskey KD, McHale NG, Thornbury KD. Role of IP3 in modulation of spontaneous activity in pacemaker cells of rabbit urethra. American Journal of Physiology - Cell Physiology 2001; 280: 1349-1356. Sergeant GP, Hollywood MA, McHale NG, Thornbury KD. Spontaneous Ca2+ activated C1- currents in isolated urethral smooth muscle cells. Journal of Urology 2001; 2001: 1161-1166. Toland HM, McCloskey KD, Thornbury KD, McHale NG, Hollywood MA. Ca2(+)-activated C1- current in sheep lymphatic smooth muscle.
American Journal of Physiology - Cell Physiology 2000; 279: C1327-C1335. Hollywood MA, McCloskey KD, McHale NG, Thornbury KD. Characterization of outward K+ currents in isolated smooth muscle cells from sheep urethra. American Journal of Physiology - Cell Physiology 2000; 279: 420-428. Sergeant GP, Hollywood MA, McCloskey KD, Thornbury KD, McHale NG. Specialised pacemaking cells in the rabbit uretha. Journal of Physiology London 2000; 526: 359-366. McHale NG, Thornbury KD, Hollywood MA. 5-HT inhibits spontaneous contractility of isolated sheep mesenteric lymphatics via activation of 5-HT4 receptors. Microvascular Research 2000; 60: 261-268. McCloskey KD, Toland HM, Hollywood MA, Thornbury KD, McHale NG. Hyperpolarization-activated inward current in isolated sheep mesenteric lymphatic smooth muscle. Journal of Physiology 1999; 521: 201-211. Hollywood MA, Cotton KD, McHale NG, Thornbury KD. Enhancement of Ca2+-dependent outward current in sheep bladder myocytes by evans blue dye. Pflugers Archiv - European Journal of Physiology 1998; 435: 631-636. Hollywood MA, Cotton KD, Thornbury KD, McHale NG. Tetrodotoxin-sensitive sodium current in sheep lymphatic smooth muscle. Journal of Physiology 1997; 503: 13-20. Cotton KD, Hollywood MA, McHale NG, Thornbury KD. Outward currents in smooth muscle cells isolated from sheep mesenteric lymphatics. Journal of Physiology 1997; 503: 1-11. Cotton KD, Hollywood MA, McHale NG, Thornbury KD. Ca2+ current and Ca2+-activated chloride current in isolated smotth muscle cells of the sheep urethra. Journal of Physiology 1997; 505: 121-131. Hollywood MA, Cotton KD, Thornbury KD, McHale NG. Modulation by cibacron blue of Ca2+ dependent K+ current in isolated smooth muscle cells from the sheep bladder. Irish Journal of Medical Science 1996; 165: 307-308. Cotton KD, Hollywood MA, Thornbury KD, McHale NG. Effect of purinergic blockers on outward current in isolated smooth muscle cells of the sheep bladder. American Journal of Physiology - Cell Physiology 1996; 270: c969-c973. Cotton KD, Hollywood MA, McHale NG, Thornbury KD. Enhancement by reactive blue 2 of outward current in isolated smooth muscle cells of the sheep bladder. Irish Journal of Medical Science 1996; 165: 307-307.   Grant Income   2001-2004 - Action Research – Physiology and Pharmacology of human urethral smooth muscle.The purpose of this study is to investigate the ionic mechanisms underlying the control and modulation of tone in the human urethra. 2002-2003 -Research into Aging Incontinence Research Grant- Ionic mechanisms underlying pacemaking in the whole isolated rat urethra. The role of this study is to develop an in vitro whole urethra preparation to investigate the contribution of ion channels and intracellular stores to urethral tone generation.
Role: Co-applicant with NG McHale, KD Thornbury & M. Shafei2002-2005 -Wellcome Trust - Pacemaker mechanisms in isolated interstitial cells of the rabbit urethraThe purpose of this study was to investigate the mechanisms underlying the generation of spontaneous inward currents in isolated IC

Completed Research Support

1998-2003 EU Fourth Framework Grant – Lymphatics and interstitium
McHale (PI)
The purpose of this study was to investigate the mechanisms underlying modulation of pacemaking in lymphatic smooth muscle during stretch.

1996-1999 British Heart Foundation- The morphological and electrical basis of pacemaking in sheep lymphatic vessels.
McHale (PI)
This study focused on examining the electrophysiology of single lymphatic smooth muscle cells and assessing the contribution of different conductances to lymphatic contractility.